Methods in Molecular Biology and Protein Chemistry: Cloning and Characterization of an Enterotoxin Subunit

The enormous advances made in molecular biology have allowed scientists to manipulate DNA with relative ease. This means that molecular biology has become a widely-used tool for answering scientific questions that may be quite unrelated to genetics and cell biology. This new book focuses on the most important techniques needed by undergraduate and post-graduate students when undertaking research in this area. Written in an accessible style and adopting a 'discovery' approach, to encourage the students to explore and experiment for them selves, this book will be invaluable to novices required and interested in learning these techniques. Students will evaluate the effect of genetic engineering through the practical application of wet biochemistry techniques and prepare mutations that can be evaluated by other classes. Coverage will include computer tutorials for sequencing and genomics and will possibly include some coverage of molecular modelling too. Guidelines on searching the primary literature, oral reports, laboratory notebooks record keeping and report writing will either be included within appendices at the back of the book, or on a supplementary web site linked to the book.