Bioanalytics : analytical methods and concepts in biochemistry and molecular biology

Unique in scope and approach, this comprehensive text on analytical methods in modern biosciences presents a thorough introduction into the physical and chemical background as well as a discussion of the strengths and weakness for each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids such as DNA and RNA. By its carefully coordinated method descriptions and frequent cross-references showing the connections between different techniques, the reader will gain a thorough overview over the entire subject and will be able to select the most appropriate method for any given bioanalytical challenge, making the text an invaluable resource for all students and researchers tasked with setting up and evaluating experimental research. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Optical spectroscopy - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic techniques - Gel and capillary electrophoresis - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - NMR and EPR of biomolecules - X-ray structure analysis - Carbohydrate analysis - Lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Genomic analysis - Proteome and metabolome analysis - Topnomics, interactomics and chemical biology