Practical Guide to Protein and Peptide Purification for Microsequencing

Why a Second Edition?The Second Edition provides practical answers to the general question, "e;How can I obtain useful sequence information from my protein or peptide?"e; rather than the more specific question asked in the first edition, "e;How can I obtain the N-terminal sequence?"e; Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.Mass spectrophotometric characterization of proteins and peptidesN-terminal sequencing of proteins with blocked N terminiInternal amino acid sequence analysis after protease digestion in-gel and on-blotImproved microscale peptide purification methodsComputer analysis of protein sequencesNew protocols tested and refined through everyday use in authors' laboratoriesUpdated reference chapter covering all aspects of protein microsequencing