Optical Microscopy : Emerging Methods and Applications

Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advancesCaged compounds, fluorescence ratio imaging, and CCD video camerasSimultaneous multiple detection and real-time fluorescence microscopySimultaneous DIC and quantitative LLF video imagingTotal internal reflectance, time-resolved, and automated fluorescence microscopyLaser-scanning confocal microscopyImaging for calcium measurements, membranes, glycoproteins, living cells, and cancer cells