Genetic Engineering: Principles, Procedures and Consequences

GENETIC ENGINEERING: Principles, Procedures and Consequences provides concise yet complete knowledge on the manyaspects of the most useful yet the most controversial scientific techniques developed under the umbrella of genetic engineering,including detection, isolation and quantitation of DNA and RNA; enzymes of recombinant DNA technology and genome editing;cloning vectors; DNA cloning; DNA libraries; identification of gene of interest; analysis of cloned gene; plant marker gene systems;gene transfer through natural means; vectorless methods of gene transfer; gene transfer methods in plants; gene transfer methods inanimals; assessment of gene transfer methods; RNA interference technology in designing transgenic crops; chloroplast geneticengineering; mitochondrial engineering; metabolic engineering; clean gene technology; genetic use restriction technology; mappingand cloning of genetic determinants of QTLs; applications and benefits of genetic engineering; Issues and disadvantages of geneticengineering; risk assessment of genetically engineered organisms; and some recent approaches, namely, genome engineering andgenomically recoded organisms, currently being developed for production of safer genetically modified organisms.This book is primarily designed as a text book for undergraduate and graduate students studying genetic engineering in agricultural,veterinary and medicinal universities. Teachers and researchers in any discipline of life sciences, agricultural sciences, medicine,and biotechnology in all the conventional and agricultural universities, research institutes, molecular genetics and biotechnologycompanies/colleges/schools all over the world will also find it useful as a reference book.SALIENT FEATURES OF THE BOOKiC* A brief historical background in every chapteriC* Nobel Prize winning work finds a special mentioniC* Simple and lucid languageiC* Supported by 23 tables, 246 figures, and more than 1,600 referencesiC* Terms defined in context of the present day knowledgeiC* 62-page glossary of more than 850 important terms related to genetic engineeringiC* Thought provoking problems at the end of each chapter to test comprehensioniC* Detailed subject indexiC* Applications/implications of various discoveries pin-pointediC* Includes "NIH Guidelines for Research involving Recombinant or Synthetic Nucleic Acid Molecules (November 2013)"as an AppendixiC* Useful of national and international level competitive examinations, entrance tests, and interviews