Vnekletochnaya Mtdnk Kak Biomarker Otsenki Luchevoy Reaktsii Organizma

Rezul'taty issledovaniya radiatsionnogo mutageneza mtDNK i izmeneniya urovnya mutantnykh kopiy mtDNK v tkanyakh obluchennykh zhivotnykh v postradiatsionnoy period sushchestvenno dopolnyayut znaniya o molekulyarnykh mekhanizmakh razvitiya luchevoy reaktsii organizma. Poluchennye rezul'taty i ispol'zovannye v rabote metodicheskie podkhody mogut byt' primeneny v dal'neyshikh eksperimental'nykh issledovaniyakh i v klinicheskoy praktike pri radioterapii opukholey. Vazhnym rezul'tatom, orientirovannym na prakticheskoe ispol'zovanie, yavlyaetsya obnaruzhenie povyshennogo urovnya mutantnykh kopiy vk-mtDNK i uvelicheniya soderzhaniya obshchey mtDNK v plazme obluchennykh zhivotnykh. Povyshennoe soderzhanie mutirovannykh vk-mtDNK v plazme krovi obluchennykh myshey svidetel'stvuet ob ikh aktivnoy eliminatsii iz kletok tkaney v postradiatsionnyy period. Poskol'ku mtDNK yavlyaetsya bolee uyazvimoy mishen'yu (chem yaDNK) dlya II i drugikh genotoksicheskikh agentov, to povyshennoe soderzhanie vk-mtDNK s mutatsiyami v plazme krovi myshey posle radiatsionnogo vozdeystviya mozhno rassmatrivat' kak potentsial'nyy biomarker dlya otsenki radiatsionnogo porazheniya i nalichiya genotoksicheskogo gruza.