Okislitel'naya Fotokonversiya Fluorestsentnykh Belkov

Fluorestsentnye belki (FB) - odin iz samykh shiroko ispol'zuemykh instrumentov v molekulyarnoy biologii. Blagodarya unikal'nomu khromoforu etikh belkov, obrazuyushchemusya avtokataliticheski iz aminokislot polipeptidnoy tsepi, geny FB mogut byt' uspeshno ekspressirovany v lyuboy biologicheskoy sisteme. Uzhe sozdana tselaya palitra fluorestsentnykh markerov s raznymi spektral'nymi svoystvami, geneticheski kodiruemye sensory dlya razlichnykh metabolitov i fiziologicheskikh parametrov, fotopereklyuchaemye belki i fotosensibilizatory. Vazhnost' otkrytiya i primeneniya FB byla otmechena v 2008 godu Nobelevskoy premiey, vruchennoy Osame Simomure, Martinu Chalfi i Rodzheru T'enu. Khotya fluorestsentnye belki semeystva GFP issledovany ochen' podrobno, v etoy oblasti est' eshche neizuchennye voprosy. Tak, nedavno v laboratorii biofotoniki IBKh RAN bylo otkryto yavlenie okislitel'noy fotokonversii, svoystvennoe nekotorym zelenym fluorestsentnym belkam. Okazalos', chto dannye belki (v tom chisle shiroko ispol'zuemyy v laboratoriyakh EGFP) mogut byt' svetoindutsiruemymi donorami elektronov. V nastoyashchey rabote my issledovali novye zakonomernosti etogo fenomena kak v usloviyakh in vitro, tak i v zhivykh kletkakh.