Analysis of Salicylates Using Maldi-MS and LC-Esi-MS in Primulae Radix : Standardization 'Key to reduce the cost of Natural Drugs'

Plants and their secondary metabolites are the rich source of drugs against several diseases. Therefore, our aim of study was to characterize, standardize and to develop a fast and sensitive matrix free Material Enhance Laser Desorption Ionization Mass Spectrometry (mf-MELDI-MS) and High Performance Liquid Chromatography-Diode Array Detection-Electrospray Ionization Mass Spectrometry (HPLC-DAD-ESI-MS) method for the qualitative and quantitative determination of salicylic acid (SA), 3,4-dihydroxybenzoic acid (3,4-DHBA), gallic acid (GA) and gallic acid methyl ester (GAME) from crude plant extract of Primulae radix.