Regeneration of Multiple Shoot Induction in Cajanus Cajan : Regeneration of Cajanus cajan LRG 30 Variety Explants on MS medium substituted with TDZ and BAP

Pigeonpea is an important pulse crop which need attention on development of efficient plant regeneration as it is a prerequisite for development of transgenic plants by in vitro Regeneration through multiple shoot inducton of different explants such as cotyledon, cotyledonary node, petiole and leaf were studied on different concentrations of TDZ and compared with best concentrations of BAP studied earlier. LRG 30 variety of Pigeonpea employed in this study. All explants were grown on MS medium substituted with TDZ (0.2, 0.4 1 & 2 mg/l) and BAP (1 & 2 mg/l). Organogenesis and embryogenesis were observed in the sections taken from callus grown on all the concentrations. Balance of endogenous of level of hormones of different explants and exogenous supply produced different response with respect to number of multiple shoots produced.