In Vitro Micropropagation and Apocarotenoid Gene Expression in Saffron : Investigation of in vitro micropropagation and apocarotenoid gene expression in perianth of saffron (Crocus sativus L.)

The oldest evidence about utilization of Saffron dates back to 2,000-1,500 years BC. Saffron is a triploid sterile species that is vegetatively propagation with Corm that only survives for only one season,reproducing via division into "cormlets" that eventually give rise to new plant and took time at least one year for each plants ,Each saffron flower has three stigmata and one stigma weights about 2mg. It takes 150,000-200,000 flowers and over 400 h of hand labor to produce 1kg saffron stigma.Now a days by studying in RT-PCR,Real Time PCR,tissue culturing,... tecniques with the help of Molecular science and biotechnology researchs we can produce Saffron widley in laboratory any time we need free of contaminants.Here,in this book Mandana Mirbaksh and Dr.Faraz Zandiyeh reviewed on important issues and published their investigation on saffron plants,its morphology,distribution,growth,medical uses,Implication of Carotenoid Biosynthesis Genes during stigma development,...They have also represented their molecular researchs in RNA extraction,cDNA preparation,RT-PCR tecnique and caught significant results.