Ethanol Production from Saccharomyces Cerevisiae : Isolation, Purification and immobilization of enzyme for enhancing ethanol production.

Ethanol is an important industrial solvent and a chemical feedstock being used for the synthesis of pharmaceuticals, detergents, adhesives, plastics, plasticizers and host for other chemicals. To reduce the cost of ethanol production, significant improvements in ethanol production technology are needed. The potential advantages of immobilized whole cell system have been recognized in the fermentation of a wide variety of carbohydrates. Immobilized cell technology has many advantages over conventional systems .This study is aimed at cell immobilization process, isolation, purification and immobilization of alcohol dehydrogenase(ADH) on different matrices from cells of S.Cerevisiae for enhancing ethanol production. The cells of Saccharomyces Cerevisiae were immobilized on different matrices viz polacrylamide, k-carrageenan, agar and calcium-alginate .Ethanol Production was improved by immobilized Alcohol Dehydrogenase and further enhanced when cell free extract was co-immobilized with isolated ADH