DNA Fingerprinting, Morphological Identification of Some Maize Lines

Two field experiments were conducted out during of 2012 and 2013 seasons at the Experimental Station Farm, Fac. of Agric., Mansoura Univ., Egypt to evaluate the morphological (quantitative and qualitative) characteristics and RAPD - PCR for identifying six genotypes of maize and their F1 single crosses. UPOV, 2009 technique was used to evaluate the genetic purity of maize genotypes and their F1 single crosses. These genotypes were: R 39, P 97, B 73, Inb.1021, Inb.1004 and Oh. 43. Morphological and bio-chemical traits used to identification genotypes of maize plants. When identification between genotypes, morphological methods using firstly, followed by the modern bio-chemical methods, especially when differences among morphological characteristics unclear. The technique polymerase chain reaction integrated is considered as effective system to identification genotypes. Thus, this study recommends using the PCR technique, despite the high cost.