Identification and Characterization of Antigens of Malarial Parasites : Identification and characterization of specific antigens of Plamodium falciparum and P. vivax using monoclonal antibodies

This book contains procedures for culture of Plasmodium falciparum in vitro, synchronization, enumeration Infected RBC, parasite antigen preparation, development of monoclonal antibodies to malarial antigens by Hybridoma, Screening procedures-IFA and ELISA and affinity purification of antigens of P. falciparum. The work also includes procedures for induction, detection and purification of plasmodium vivax fusion proteins expresssed in lamda gt11 and pMAL vectors.