In Vitro Maturation and Fertilization of Oocytes in Goat : EFFECT OF COLLECTION TECHNIQUES AND FOLLICULAR FLUID ON IN VITRO MATURATION AND FERTILIZATION OF OOCYTES IN GOAT

In vitro maturation (IVM) and fertilization (IVF) is the basic requirement of in vitro production (IVP) of embryos. The cumulus cells (CCs) surrounding the oocyte plays a key role of cytoplasmic maturation. It supplies nutrients, energy substrates and/or messenger molecules for the development of oocyte. Oocyte collection techniques have a significant role in IVM. Maturation media also plays a vital role on developmental potential of oocytes in IVP procedure. To establish a well-defined medium different serum have been extensively supplemented to the basic medium. Follicular fluid after necessary treatments could also be used as an economic supplement in the culture media (TCM-199).