Biotoxicity Assay of Bacillus Thuringiensis Against T.Castaneum : Bacillus thuringiensis, Crystal Protein, Cry 33, Assay, Probit, LC50, Infestation, Flour beetle, Pesticides

The study conducted on the toxic effect of the Bacillus thuringiensis spores against the Tribolium castaneum can be of great importance in eliminating the stored pest grains. There are a lot of future perspectives of this study which are given below: Proper processes for the collection and handling of the samples should be under consideration in order to avoid the incorrect and contaminated samples from different sources. According to the nutritional requirements of the Bacillus thuringiensis a specific solid media called as T3 media is used for the culturing of these bacteria and incubation period of these is 72 hours for spore formation. To understand the physiology of the Tribolium castaneum is quite helpful in understanding the rearing processes of these beetles. Biotoxiciy assay provides a reliable method for determining the susceptibility of the Tribolium castaneum against Bacillus thuringiensis that provide an easy method to determine the mortality ratio. The spores of the Bacillus thuringiensis can be of great importance in the agriculture in reducing the considerable population of the stored grain pest such as Tribolium castaneum and also some other pests.