Bacterial Glucose Oxidase : Physiology of Glucose oxidase production in Pseudomonas aeruginosa and other Gram Negative Bacteria

Enzyme is a protein biocatalyst produced by living cells. They bring about & control the numerous biochemical reactions involved in metabolic process of cells.They are widely applied in research, industry & diagnostic purposes. In this book production of such commercially & diagnostically important enzyme Glucose Oxidase is discussed whichbelongs to the class-oxidoreductase. Glucose oxidasehas a large demand all over the world. Currently it is produced from Asp.niger &Penicillium notatum. In our laboratory bacterial source of this enzyme is isolated, which is Pseudomonas aeruginosa from paper mill waste. The production at laboratory scalehas been standardized. The strain is deposited atNational Collection Ind.& Marine Bacteria (NCIMB) Abberdeen U.K.with accession number 13118. The strain was mutated with UV-rays &EMS. The mutant strain showed 40 percent enzyme activity extracellular. The other characters were also studied. This is a novel bacterial source of glucose oxidase.