Biotechnology for Toxicity Evaluation of Pest Control Agents : Evaluation of Pesticides Immunotoxicity and their Mixtures by modern techniques

Chemical pesticides and bioinsecticide alone or there mixture, are immunotoxic to earthworm, fish, and mice. The study consisted of three parts: A- the development of a new ultrasound protocol for extrusion of coelomocytes from the earthworm, B- comparative cellular responses of earthworm coelomocytes, of different ages and from different worm species,exposed in vitro to the tested pesticides; C- evaluation of the immunotoxicity of the mixture of the tested pesticides on three animal models (earthworm, Eisenia fetida; trout fish, and inbred female C57BI/6 mice , From an immunological point of view, the chemical pesticides mixture, at the tested levels may not pose a grater risk to the immune system in fish and mic